Fig. 6.

S100B expression in Olig2-null and Myrf conditional knockout mutants. a, b S100B+ cells were dramatically reduced in E18.5 Olig2-null spinal cord in the white matter, but not in the gray matter. c–j The number of S100B+/SOX10+ oligodendrocytes was significantly reduced in the white matter of spinal cord and forebrain (corpus callosum) in Myrf conditional knockout mutants. k–l Statistical analysis of the number of S100B+ oligodendrocytes in Olig2 and Myrf mutants. SOX10+/S100B+ cells are represented by white arrows. m Schematic diagram of the time window of S100B expression in differentiating oligodendrocytes and gray matter astrocytes. In oligodendrocyte lineage, S100B expression is slightly earlier than that of MYRF, and gradually down-regulated in ASPA+ mature oligodendrocytes. In astrocyte lineage, S100B is gradually up-regulated and maintained in adulthood. sc spinal cord, WM white matter, GM gray matter, CC corpus callosum, OLs oligodendrocytes, OPCs oligodendrocyte progenitor cells, RGCs radial glial cells. n = 3, *p < 0.05, **p < 0.01. Bar, 100 μm