. 2021 Jun 7;11(12):7327–7338. doi: 10.1021/acscatal.1c00847

Table 2. Biochemical Characterization of the MthUPO Wild Type and the Evolved Variants toward the Substrate NBD^a.

MthUPO variant	K_m [μM]	k_cat [s^–1]	k_cat/K_m [M^–1 s^–1]
WT	386	7.1	1.9 × 10⁴
L60F	110	2.9	2.7 × 10⁴
L60F/S159G/A161F	422	132.2	3.1 × 10⁵
F59Q/L60M/S159G/F154A	290	59.4	2.1 × 10⁵
F59Q/L60F/S159G	303	47.0	1.6 × 10⁵

Standard deviation for the kinetics <18%, values were calculated with the corrected extinction coefficient of 10,870 M^–1 cm^–1. For reaction conditions, see the Materials and Methods. For Michaelis–Menten plots, see the Supporting Information.

Table 2. Biochemical Characterization of the MthUPO Wild Type and the Evolved Variants toward the Substrate NBDa.

Table 2. Biochemical Characterization of the MthUPO Wild Type and the Evolved Variants toward the Substrate NBD^a.