Table 3. Catalytic Activity of MthUPO Variants toward Benzylic Hydroxylation Yielding Chiral Products^a.

^aTON = turnover number, standard deviation <6.5%, reaction conditions: 100 nM MthUPO variant, 1 mM indane, 1 mM H₂O₂, 100 mM KPi buffer (pH 7), 5% acetone (v/v), 1 h at 25 °C in triplicates.

^b100 nM MthUPO variant, 1 mM 1,2,3,4-tetrahydronaphthalene, 1 mM H₂O₂, 100 mM KPi buffer (pH 7), 5% acetone (v/v), 1 h at 25 °C in triplicates.

^c100 nM MthUPO variant, 1 mM 1,2,3,4-tetrahydronaphthalene, 2 mM H₂O₂, 100 mM KPi buffer (pH 7), 5% acetone (v/v), 2 h at 25 °C in triplicates.

^dDetermined by chiral GC.