Figure 2.

Identification of significantly enriched drivers and cell types

(A) Growth kinetics of round 1 teratoma formation for injections with driver library transduced hESCs vs control WT hESCs.

(B) UMAP visualization of cell types from round 1 teratomas formed by driver library transduced hESCs.

(C) Growth kinetics of round 2 tumors formed from re-injected cells from round 1 teratomas formed by driver library transduced hESCs vs WT hESCs. Control measurements are from a common set of tumors grown from the parent WT hESC line, which were used as growth controls for all experiments in this study.

(D) UMAP visualization of cell types from round 2 tumors formed by re-injected cells from round 1 teratomas of driver library transduced hESCs.

(E) Relative fraction of each cell type in round 1 teratomas formed from library transduced hESCs and WT hESCs, and log fold change with associated -log(p-value) of each cell type for driver library teratomas vs WT teratomas.

(F) Relative fraction of each cell type in round 2 tumors formed from library transduced hESCs and WT hESCs, and log fold change with associated -log(p-value) of each cell type for driver library tumors vs WT tumors.

(G) Relative fraction of top enriched drivers prior to injection and in each round of tumor formation.

(H) H&E stained sections of round 2 tumors formed from WT and driver library transduced hESCs. WT tumors display mature cell types from all three germ layers, such as cartilage (top right), muscle (bottom right) and dermis-like epithelium (bottom left). Driver library tumors display disorganized and more homogeneous composition along with markers of transformation such as nuclear pleomorphism (top right), areas of high mitotic rates (bottom right) and areas of necrosis (bottom left). Scale bars for full sections are 5 mm, scale bars for magnified images are 50 μm.