Table 2.
Antibodies for FACS and histology
| Type | Host | Target | Conjugation | Manufacturer | ID |
|---|---|---|---|---|---|
| Primary | Rabbit | TMEM119 | NA | Abcam | ab209064 |
| Goat | IBA1 | NA | Abcam | ab48004 | |
| Rabbit | IBA1 | NA | Wako | 019–19741 | |
| Mouse | APOE | NA | Abcam | ab1907 | |
| Rabbit | IGF1 | NA | Abcam | ab40657 | |
| Rabbit | CST7 | NA | Nordic Biosite | 12073–1-AP | |
| Secondary | Donkey | Rabbit | Alexa Fluor 488 | Abcam | ab150073 |
| Donkey | Mouse | Alexa Fluor 488 | Abcam | ab150105 | |
| Donkey | Rabbit | Alexa Fluor 594 | Abcam | ab150076 | |
| Donkey | Mouse | Alexa Fluor 594 | Abcam | ab150108 | |
| Donkey | Goat | Alexa Fluor 594 | Abcam | ab150132 | |
| Donkey | Rabbit | Alexa Fluor 647 | Abcam | ab150075 | |
| Donkey | Goat | Alexa Fluor 647 | Abcam | ab150135 | |
| Preconjugated | Rat | CD11B | PerCP-Cy5.5 | Biolegend | 101227 |
| Rat | CD11B | APC | BD Biosciences | 553312 | |
| Rat | CD11B | BV421 | BD Biosciences | 562605 | |
| Rat | CD45 | PE/Cy7 | Biolegend | 103113 | |
| Rat | CD45 | PE | BD Biosciences | 553081 | |
| Rat | LY6C | PE | Biolegend | 128007 | |
| Rat | LY6G | BV421 | BD Biosciences | 562737 | |
| Rat | LY6G | V450 | BD Biosciences | 560603 | |
| Rat | CD19 | BV421 | BD Biosciences | 562701 | |
| Rat | SIGLEC-F | BV421 | BD Biosciences | 562681 | |
| Hamster | CD3E | BV421 | BD Biosciences | 562600 | |
| Mouse | NK1.1 | BV421 | Biolegend | 108741 | |
| Rat | CD14 | FITC | Biolegend | 123307 | |
| Mouse | CD64 | PerCP-Cy5.5 | Biolegend | 139308 | |
| Live/dead | NA | Free amines | Near-IR | Thermo Fisher | L34959 |
| Counterstain | NA | dsDNA | Hoechst 33258 | Invitrogen | H3569 |
| Fc block | Rat | CD16/32 | NA | BD Biosciences | 553141 |
NA: Not Applicable.