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. 2021 Oct 6;41(40):8309–8320. doi: 10.1523/JNEUROSCI.0711-21.2021

Figure 3.

Figure 3.

CDK-14 interacts with MIG-5. A, Wnt signaling pathway. Wnt ligand binds to the Fz receptor and activates Dsh. Schematic diagrams of C. elegans Dsh proteins (MIG-5, DSH-1, and DSH-2) are shown. The km91, rh147, and ok1445 mutation sites are indicated. The region deleted in ok2162 is indicated by black bars. DAX, Domain present in Disheveled and axin; PDZ, PSD-95, Dlg, and ZO-1/2 domains; DEP, Disheveled, Egl-10, and pleckstrin domains. B, Interaction of CDK-14 with MIG-5b. COS-7 cells were transfected with plasmids encoding FLAG-CDK-14, GFP-MIG-5b, and GFP-MIG-5b(311−666), as indicated. Total lysates and immunoprecipitated complexes obtained with anti-GFP antibody [immunoprecipitation (IP): GFP] were analyzed by immunoblotting (IB). Schematic diagrams of MIG-5b and MIG-5b(311−666) are shown above. C, Percentage of axons that initiated regeneration 24 h after laser surgery in young adults. The number of axons examined is shown. Error bars indicate 95% confidence intervals. NS, Not significant. D, Interactions of CDK-14 with DSH-1 and DSH-2. COS-7 cells were transfected with plasmids encoding FLAG-CDK-14, GFP-MIG-5b, GFP-DSH-1, and GFP-DSH-2, as indicated. Total lysates and immunoprecipitated complexes obtained with anti-GFP antibody (IP: GFP) were analyzed by IB.