Figure 6.

EPHX-1/RhoGEF functions between CDK-14 and CDC-42 GTPase in the axon regeneration pathway. A, Structures of Dbl family proteins. Human WGEF interacts with Dsh in the Wnt signaling pathway. Schematic diagrams of C. elegans EPHX-1, human WGEF, and human ephexin-1 are shown. B, Structure of the ephx-1 gene. Schematic diagrams of EPHX-1 isoforms are shown. The ephx-1 gene encodes four isoforms, EPHX-1a, EPHX-1b, EPHX-1c, and EPHX-1d. Boxes indicate exons, and lines indicate introns and untranslated regions. The yellow, green, and red boxes represent the DH, PH, and SH3 domains, respectively. The regions deleted in tm11319 and ok494 alleles are indicated by black bars. C, E, Percentages of axons that initiated regeneration 24 h after laser surgery in young adults. The number of axons examined is shown. Error bars indicate 95% confidence intervals. *p < 0.05, **p < 0.01, ***p < 0.001, as determined by Fisher's exact test. NS, Not significant. D, Length of regenerating axons 24 h after laser surgery. Data are presented as a boxplot representing the median (thick line within the box) and interquartile range (edge of box) with individual data points. The number (n) of axons examined is shown. Statistical significance was determined by the Wilcoxon rank-sum test.