Figure 4.

Arrestin-1 bidirectional translocation defect and light avoidance in cKO mice. A, Kinetics of arrestin-1 translocation during light and dark adaptation: immunostaining of retinal sections of P50 mice showing arrestin-1 distribution 0, 10, 20, 30, 60, and 120 min after light onset (light 900 lux) and 0, 10, 20, 30, 60, and 120 min after light offset in control Otx2^flox/flox (upper panel) and cKO Crx-CreERT2;Otx2^flox/flox (lower panel) mice injected with tamoxifen at P30. B, Light dark box test. Histograms represent the time spent (in seconds) in the light compartment in three conditions: with an attractive object in the light box (object in light box), with an attractive object in the dark box (object in dark box), or without any motivation (without object) in control (n = 10) and cKO (n = 13) mice (*p < 0.05, **p < 0.01, ***p < 0.005, t test). C, Openfield test. Histograms represent the total time spent (in seconds) in the periphery of the openfield (n = 13; *p < 0.05, **p < 0.01, ***p < 0.005, t test). OS, outer segments; IS, inner segment; ONL, outer nuclear layer. Scale bar: 25 μm.