Figure 5.

Whole-body knockdown of CG4607 is lethal upon HCD feeding. Survival of ubi-GAL4>CG4607^KD flies on NF and HCD. (A) Adult male ubi-GAL4>CG4607^KD flies (n = 60) were placed on either NF or HCD and manually monitored for lethality every 24 hours. (B) Actogram of activity (n = 16 flies) wrapped over 24 hours of single housed individual control and ubi-GAL4>CG4607^KD flies in DAMS monitors and beam crosses were monitored every 5 minutes while feeding on NF or HCD (C) The same actogram as in (B), but the activity between 6 and 9 hours is shown. (D) A box plot of the mean activity (beam crosses from 0 to 6 hours) of control and ubi-GAL4>CG4607^KD flies fed NF or HCD. Kruskal-Wallis test was used to calculate significance. (E) Caloric intake of adult male flies (n = 50/genotype) using the CAFE assay. Data are mean ± SD and significance between genotypes (*) and diets (#) were calculated using a t-test, **P < 0.01, #### P < 0.0001 (Prism).