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. 2021 Apr 26;11(7):jkab134. doi: 10.1093/g3journal/jkab134

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Published by Oxford University Press on behalf of Genetics Society of America 2021. This work is written by US Government employees and is in the public domain in the US.

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

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sch9Δ mutant cells in the W303 background have growth defects and rapidly accumulate suppressor mutations. (A) Tetrad analysis of wild type (W303-1A) × sch9Δ (PPY624) on YPD plate medium. Each tetrad, numbered 1-4, was dissected into four spores labeled A-D. (B) sch9Δ mutants quickly accumulate suppressor mutations. An sch9Δ mutant strain (PPY624) was streaked on YPD plate. Small white colonies are sch9Δ mutants, while large red colonies are sch9Δ mutants carrying suppressor mutations. (C) The growth defects of sch9Δ mutants are suppressed by a sns1 mutation. Wild type (W303-1A), sch9Δ (PPY624), and sch9Δ sns1 (PPY673) mutant strains were serially diluted and spotted on YPD (Dextrose), YPL (Lactate), and YPEG (Ethanol/Glycerol) medium.