Table 1.
Yeast strains used in this study
| Strain | Genotype | Source | Application |
|---|---|---|---|
| BY4741 | MATa his3Δ1 leu2Δ0 met17Δ0 ura3Δ0 | Lab. stock | Figures 2B, 3, 4, 5, 6C, and 7 |
| BY4742 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | Lab. stock | Figure 2A |
| W303-1A | MATa ura3-1 ade2-1 leu2-3,112 trp1-1 his3-11,15 | Lab. stock | Figures 1 and 3D |
| W303-1B | MATα ade2-1, his3-11, 15, ura3-1, leu2-3, 112, trp1-1, can1-100 | Lab. stock | Figure 3D |
| PPY566 | BY4741 sch9Δ::kanMX4 sns1 | This study | Figures 2A and 3B |
| ZLY6649 | BY4741 sch9Δ::kanMX4 | This study | Figures 2B, 4D, 5B, and 5C |
| ZLY6650 | BY4741 sch9Δ::kanMX4 | This study | Figures 4C, 4E, 5C, and 8 |
| PPY730 | BY4741 sns1 | This study | Figures 2B and 3B |
| PPY733 | BY4741 sch9Δ::kanMX4 sns1 | This study | Figures 2B and 5D |
| PPY632 | BY4741 sch9Δ::kanMX4 | This study | Figures 3B, 4F, and 5E |
| PPY633 | BY4742 sch9Δ::kanMX4 | This study | For constructing PPY745 |
| PPY731 | BY4742 sns1 | This study | For constructing PPY737 |
| PPY734 | BY4742 sch9Δ::kanMX4 sns1 | This study | For constructing PPY741 |
| BY4743 | BY4741 × BY4742 | This study | Figures 2C, 3, 4A, 5, and 8 |
| PPY745 | PPY632 × PPY633 | This study | Figures 2C, 3, 4A, 5A, 5D, and 8 |
| PPY737 | PPY730 × PPY731 | This study | Figures 2C, 3, 4A, and 8 |
| PPY741 | PPY733 × PPY734 | This study | Figures 2C, 3, 4A, 5A, and 8 |
| PPY624 | W303-1B MATα sch9Δ::kanMX4 | This study | Figures 1 and 3D |
| PPY673 | W303-1A MATa sch9Δ::kanMX4 sns1 | This study | Figure 1 |
| PPY622 | W303-1A MATa sch9Δ::kanMX4 | This study | Figure 3D |
| ZLY6614 | BY4741 sns2 | This study | Figures 2B, 3C, and 4A |
| ZLY6619 | BY4741 sch9Δ::kanMX4 sns2 | This study | Figures 2, A, B, 3C, 4A, and 5D |
| ZLY6626 | BY4742 sch9Δ::kanMX4 sns2 | This study | Figures 4A and 5D |
| ZLY6581 | BY4742 sns2 | This study | Figure 4A |
| ZLY6519 | BY4741 isc1Δ::kanMX4 | This study | Figure 4B |
| ZLY6606 | BY4741 mck1Δ::kanMX4 | This study | Figure 4, B–F |
| ZLY6609 | BY4741 ira2Δ::kanMX4 | This study | Figure 5, B and E |
| ZLY6629 | BY4741 get2Δ::kanMX4 | This study | Figure 4B |
| ZLY6634 | BY4742 vma6Δ::kanMX4 | This study | Figure 4B |
| ZLY6633 | BY4742 paf1Δ::kanMX4 | This study | Figure 4B |
| ZLY6637 | BY4741 atg15Δ::kanMX4 | This study | Figure 4B |
| ZLY6640 | MATα his3Δ1 leu2Δ0 ura3Δ0 met17Δ0 def1Δ::kanMX4 | This study | Figure 4B |
| ZLY6642 | BY4742 tps2Δ::kanMX4 | This study | Figure 4B |
| ZLY6644 | BY4741 dep1Δ::kanMX4 | This study | Figure 4B |
| ZLY6645 | BY4742 sem1Δ::kanMX4 | This study | Figure 4B |
| ZLY6647 | BY4741 ira1Δ::kanMX4 | This study | Figure 5E |
| ZLY6662 | BY4741 vac8Δ::kanMX4 | This study | Figure 4B |
| ZLY6665 | BY4742 erv14Δ::kanMX4 | This study | Figure 4B |
| ZLY6669 | BY4741 vma2Δ::kanMX4 | This study | Figure 4B |
| ZLY6604 | BY4741 gpb2Δ::kanMX4 | This study | Figures 4, B, C, E, and F |
| ZLY6660 | MATa his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 met17Δ0 sch9Δ::kanMX4 ira2Δ::kanMX4 | This study | Figure 5E |
| ZLY6661 | BY4741 sch9Δ::kanMX4 ira2Δ::kanMX4 | This study | Figure 5, B and D |
| ZLY6601 | BY4742 ira2Δ::kanMX4 | This study | Figure 5B |
| ZLY6648 | BY4742 ira1Δ::kanMX4 | This study | Figure 5C |
| ZLY6651 | BY4742 sch9Δ::kanMX4 | This study | Figure 4C |
| ZLY6602 | BY4742 gpb2::kanMX4 | This study | Figure 4C |
| ZLY6652 | BY4741 sch9Δ::kanMX4 mck1Δ::kanMX4 | This study | Figure 4, D and F |
| PPY754 | MATa his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 met17Δ0 sch9Δ::kanMX4 ira1Δ::kanMX4 | This study | Figure 5C |
| PPY755 | BY4742 sch9Δ::kanMX4 ira1Δ::kanMX4 | This study | Figure 5, D and E |
| PPY734 | BY4742 sch9Δ::kanMX4 sns1 | This study | Figure 5D |
| PPY777 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 met17Δ0 sch9Δ::kanMX4 gpb1Δ::kanMX4 | This study | Figure 4F |
| PPY757 | BY4741 gpb1Δ::kanMX4 | This study | Figure 4, C, E, and F |
| ZLY6677 | BY4742 sch9Δ::kanMX4 gpb2Δ::kanMX4 | This study | Figure 4, C, E, and F |
| PPY763 | MATa his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 gpb1Δ::kanMX4 gpb2Δ::kanMX4 | This study | Figure 4, E and F |
| PPY767 | BY4741 sch9Δ::kanMX4 gpb1Δ::kanMX4 gpb2Δ::kanMX4 | This study | Figure 4, E and F |
| ZLY4313 | BY4741 yak1Δ::kanMX4 | This study | Figures 6 A, B, and 7A |
| ZLY5023 | BY4741 yak1Δ::HIS3 tpk1Δ::kanMX4 tpk2Δ::kanMX4 tpk3Δ::kanMX4 | Figure 6, A and B | |
| ZLY6715 | BY4741 bcy1Δ::kanMX4 | This study | Figure 6C |
| PPY776 | MATa his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 pde1Δ::kanMX4 pde2Δ::kanMX4 | This study | Figures 6C and 7B |
| ZLY6578 | BY4743 ira2Δ/IRA2 | Yeast genome deletion project | Figure 5B |
| ZLY6656 | BY4743 ira1Δ/IRA1 | Figure 5C | |
| ZLY6707 | BY4741 sch9Δ::kanMX4 yak1Δ::kanMX4 | This study | Figure 7A |
| ZLY4368 | BY4741 pde2Δ::kanMX4 | This study | Figure 7B |
| PPY758 | BY4741 sch9Δ::kanMX4 pde2Δ::kanMX4 | This study | Figure 7B |