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. 2021 Oct 6;9(10):e002755. doi: 10.1136/jitc-2021-002755

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© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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Design of a novel zinc-finger protein-based artificial transcription system for strict and effective gene control. (A) Diagram of transactivators and response elements used in the regulatory system. ZFP, a novel zinc-finger protein specifically targeting UAS sequence; VP64, activation domain; UASLTR, a novel response element consisting of UAS sequence inserted into upstream of minimal LTR promoter; UASCMV, UAS sequence linked to upstream of minimal CMV promoter. (B–D) Basal expression of mCherry driven by different hybrid promoters and inducible expression of mCherry by different transactivators were evaluated by fluorescence microscopy (B) and FACS (B–D) in 293 T cell transfection assay. (n=3, mean±SD is shown, **p<0.01, ****p<0.0001, analyzed using Student’s t-test). CMV, cytomegalovirus; DBD, DNA binding domain; NLS, nuclear localization signal; UAS, upstream activating sequence; ZFP, zinc finger proteins.