Figure 1.

Western blot assessment of phosphorylated Ub species. Equal masses of recombinant Ub and p-S65-Ub monomers and tetramers with different chain linkages were tested. Shown are representative western blots (top panels) and quantifications relative to silver stained gels (bottom panels). (A) Membranes were probed with four different p-S65-Ub Abs (A-D) and linkage-specific poly-Ub Abs targeting K48- or K63 chains. (B) Membranes were probed with five different total Ub Abs (1–5) (see Table 1). Western blot intensity levels were normalized to intensity levels from corresponding silver staining