Table 1.
Primary and secondary Abs used in the study
| Text identifier | Clone | Dilution WB (Final conc.) |
MSD (Final conc. in μ/ml) |
ICC/IHC (Final conc. in μg/ml) |
Host Species | Company | Catalog No. |
|---|---|---|---|---|---|---|---|
| p-S65-Ub Ab A | Polyclonal | 1:1,000 (0.24 μg/mL) |
1 | 1.9 /4.7 | Rabbit IgG | CST | 37642 |
| p-S65-Ub Ab B | Polyclonal | 1:1,000 (0.51 μg/mL) |
1 | 2.0 /0.77 | Rabbit IgG | Springer-lab | in house Ab |
| p-S65-Ub Ab C | Polyclonal | 1:1,000 (0.5 μg/mL) |
1 | 2.0 /10 | Rabbit IgG | Millipore | ABS1513 |
| p-S65-Ub Ab D | E2J6T | 1:1,000 (0.1 μg/mL) |
1 | 0.08 /0.4 | Rabbit IgG | CST | 62802 |
| Ub Ab 1 | 1D7B2 | 1:1,000 (0.65 μg/mL) |
1 | – - | Mouse IgG1 | Proteintech Group | 60310-1-01 g |
| Ub Ab 2 | P4D1 | 1:1,000 (0.2 μg/mL) |
0.2, 1, 5 μg/ml | – - | Mouse IgG1 | Santa Cruz | sc-8017 |
| Ub Ab 3 | UBI-1 | 1:1,000 (1 μg/mL) |
1 μg/ml | – - | Mouse IgG1 | Millipore | MAB1510 |
| Ub Ab 4 | VU-1 | 1:1,000 (0.5 μg/mL) |
1 μg/ml | – - | Mouse IgG1 | Life Sensors | VU-101 |
| Ub Ab 5 | FK2 | 1:1,000 (1 μg/mL) |
1 μg/ml | – - | Mouse IgG1 | Millipore | 04–263 |
| K48-Ub Ab | D9D5 | 1:1,000 | – - | – - | Rabbit IgG | CST | 8081 |
| K63-Ub Ab | D7A11 | 1:1,000 | – - | – - | Rabbit IgG | CST | 5621 |
| p-S65-PRKN | polyclonal | 1:5,000 | – - | – - | Rabbit IgG | Muqit-lab | – – |
| PRKN Ab | Prk8 | 1:2,000 | – - | – - | Mouse IgG1 | CST | 4211 |
| PINK1 Ab | D8G3 | 1:1,000 | – - | – - | Rabbit IgG | CST | 6946 |
| GAPDH Ab | – - | 1:400,000 | – - | – - | Mouse IgG1 | Meridian | H86504M |
| Anti-mouse IgG, HRP-linked |
Secondary Ab | 1:10,000 | – - | – - | Donkey IgG | Jackson IR | 715–035-150 |
| Anti-rabbit IgG, HRP-linked |
Secondary Ab | 1:10,000 | – - | – - | Donkey IgG | Jackson IR | 711–035-152 |
WB: western blot; MSD: Meso Scale Discovery; ICC: immunocytochemistry; IHC: immunohistochemistry; CST: Cell Signaling Technology; Jackson IR: Jackson ImmunoResearch Laboratories, Inc.; Santa Cruz: Santa Cruz Biotechnology