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Limit of blank, limit of detection, and limit of quantification for recombinant p-S65-Ub in blocking buffer
| p-S65-Ub antibody | linkage type, mono/tetramer | Limit of Blank (LoB) (MSD-ECL units) |
Limit of Detection (LoD) (MSD-ECL units) |
Limit of Quantification (LoQ) (CV<5%) |
|---|---|---|---|---|
| p-S65-Ub Ab D | Mono p-S65-Ub | 251 | 326.3 | 330 ng/ml (39 nM) |
| K63 p-S65-Ub4 | 251 | 276.6 | 330 pg/ml (9.7 pM) | |
| K48 p-S65-Ub4 | 251 | 256.2 | 3.3 pg/ml (97 fM) | |
| M1 p-S65-Ub4 | 251 | 266.1 | 330 fg/ml (9.7 fM) |
