TABLE 2.
Length determination (in base pairs) of the PCR fragment containing the ITS2 of known fungal isolates
| Organism | Strain(s)a | Conventional ID | PCR fragment length (bp) |
|---|---|---|---|
| Yeasts | |||
| Candida albicans | ATCC 10231, SCC, HSC1159, HSC67442, HSC67630, HSC68392, HSC81363 | API 20C-AUX | 279 |
| Candida kefyr | ATCC 4135, SCC | API 20C-AUX | 372 |
| Candida guilliermondii | CAP F1-95, SCC | API 20C-AUX | 321 |
| Candida tropicalis | ATCC 66029, SCC, HSC75317, HSC75418, HSC75422, HSC78308, HSC78408, HSC81682, HSC81701, HSC71273, HSC71278, HSC29977, HSC71277 | API 20C-AUX | 269 |
| Candida krusei | ATCC 6258, SCC, CT1, K1865, CA37, E175 | API 20C-AUX | 282 |
| Candida glabrata | ATCC 90030, SCC, HSC17253, HSC20818, HSC20819 | API 20C-AUX | 360 |
| Candida zeylanoides | CAP F18-93 | API 20C-AUX | 316 |
| Candida lusitaniae | ATCC 42720 | API 20C-AUX | 199 |
| Candida parapsilosis | ATCC 22019, SCC, HSC5966, HSC21193 | API 20C-AUX | 251 |
| Candida pelliculosa | ATCC 8168 | API 20C-AUX | 317 |
| Candida pseudotropicalis | ATCC 66028 | API 20C-AUX | 372 |
| Candida stellatoidea | ATCC 36232 | API 20C-AUX | 279 |
| Trichosporon beigelii | ATCC 28592 | API 20C-AUX | 295 |
| HSC6088 | API20C-AUX | 298 | |
| Geotrichum candidum | ATCC 34614 | API 20C-AUX, mannitol | 192 |
| Cryptococcus neoformans | ATCC 90112 | API 20C-AUX, urease, phenoloxidase | 315 |
| Cryptococcus albidus | ATCC 10666 | API 20C-AUX | 350 |
| Cryptococcus laurentii | ATCC 18803 | API 20C-AUX | 306 |
| Rhodotorula rubra | ATCC 9449, SCC HSC20116, HSC37777 | API 20C-AUX | 348 |
| Blastoschizomyces capitatus | ATCC 10663 | API 20C-AUX | 248 |
| Saccharomyces cerevisiae | ATCC 9763 | API 20C-AUX | 363 |
| Malassezia furfur | ATCC 14521 | API 20C-AUX | 494 |
| Other opportunistic fungi | |||
| Aspergillus fumigatus | CAP F6-91 | Morphology | 284 |
| Aspergillus flavus | ATCC 10124 | Morphology | 288 |
| Aspergillus niger | ATCC 16404 | Morphology | 290 |
| Aspergillus terreus | ATCC 28301 | Morphology | 292 |
| Alternaria alternata | CAP F13-93 | Morphology | 285 |
| Penicillium sp. | SCC | Morphology | 283 |
| Acremonium strictum | CAP F4-96 | Morphology | 294 |
| Fusarium solani | CAP F15-95 | Morphology | 286 |
| Paecilomyces sp. | SCC | Morphology | 287 |
| Rhizopus sp. | SCC | Morphology | 319 |
| Absidia corymbifera | ATCC 66271 | Morphology | 410 |
| Mucor sp. | SCC | Morphology | 312 |
| Cunninghamella bertholletiae | ATCC 42115 | Morphology | 347 |
| Pseudallescheria boydii | ATCC 58085 | Morphology | 321 |
| Scedosporium prolificans | SCC | Morphology | 303 |
| Phialophora verrucosa | ATCC 28181 | Morphology | 308 |
| Phialophora richardsiae | ATCC 26465 | Morphology | 276 |
| Fonsecaea pedrosoi | ATCC 28174 | Morphology | 319 |
| Cladosporium carrionii | ATCC 32279 | Morphology | 303 |
| Wangiella dermatitidis | SCC | Morphology | 334 |
| Exophiala jeanselmei | ATCC 10224 | Morphology | 311 |
| Stemphylium ilicis | ATCC 18160 | Morphology | 280 |
| Dimorphic fungi | |||
| Penicillium marneffei | ATCC 18224 | Morphology | 280 |
| Sporothrix schenckii | ATCC 10212 | Morphology, conversion to yeast phase | 310 |
| Blastomyces dermatitidis | HSC patient isolate | Nucleic acid probe | 315 |
| Histoplasma capsulatum | HSC patient isolate | Nucleic acid probe | 299 |
| Dermatophytes | |||
| Microsporum canis | CAP F4-90 | Morphology | 321 |
| Microsporum gypseum | CAP F20-90 | Morphology | 321 |
| Microsporum audouinii | ATCC 42558 | Morphology | 318 |
| Microsporum nanum | SCC | Morphology | 324 |
| Microsporum gallinae | SCC | Morphology | 325 |
| Microsporum cookei | SCC | Morphology | 314 |
| Trichophyton schoenleinii | SCC | Morphology, nutritional and temp (°C) requirements | 306 |
| Trichophyton terrestre | SCC | Morphology, nutritional and temp (°C) requirements | 298 |
| Trichophyton violaceum | SCC | Morphology | 315 |
| Trichophyton mentagrophytes | ATCC 28185 | Morphology, urease, hair performation | 303 |
| Trichophyton rubrum | ATCC 28188 | Morphology | 307 |
| Trichophyton tonsurans | ATCC 28942 | Morphology and nutritional requirements | 302 |
| Trichophyton verrucosum | CAP F15-1994, SSC | Morphology, nutritional and temp (°C) requirements | 307 |
| Epidermophyton floccosum | ATCC 52066 | Morphology | 366 |
a
SCC and HSC, stock culture collection and patient isolates, respectively, obtained from the Health Sciences Centre, Winnipeg, Manitoba, Canada.