Table 2.
Nucleotide sequences for real-time PCR primers.
| Gene | Accession Number | Primer sequence, 5′→ 3′ | Product size (bp) |
|---|---|---|---|
| IL-1β | NM_204524.1 | GGTCAACATCGCCACCTACA | 86 |
| CATACGAGATGCAAACCAGCAA | |||
| IL-6 | NM_204628.1 | CCTTTCAGACCTACCTGGAATT | 130 |
| ACTTCATCGGGATTTATCACCA | |||
| TNF-α | NM_204267.1 | TGTGTATGTGCAGCAACCCGTAGT | 229 |
| GGCATTGCAATTTGGACAGAAGT | |||
| IFNγ | NM_205149.1 | TGAGCCAGATTGTTTCGATG | 246 |
| TCCTTTTGAAACTCGGAGGA | |||
| NF-κB | XM_015285418.2 | CACGGAGGCTTGATCCTGTT | 96 |
| CCGCTGTCCTGTCCATTCTT | |||
| TLR4 | NM_001030693.1 | AGTCTGAAATTGCTGAGCTCAAAT | 190 |
| GCGACGTTAAGCCATGGAAG | |||
| ZO-1 | XM_015278981.2 | CTTCAGGTGTTTCTCTTCCTCCTCTC | 131 |
| CTGTGGTTTCATGGCTGGATC | |||
| occludin | NM_205128.1 | GCTCTGCCTCATCTGCTTCTT | 142 |
| CCCATCCGCCACGTTCTTC | |||
| claudin-1 | NM_001013611.2 | CTGATTGCTTCCAACCAG | 140 |
| CAGGTCAAACAGAGGTACAAG | |||
| claudin-2 | NM_001277622.1 | CCTGCTCACCCTCATTGGAG | 145 |
| GCTGAACTCACTCTTGGGCT | |||
| GAPDH | NM_204305.1 | GCCCAGAACATCATCCCA | 137 |
| CGGCAGGTCAGGTCAACA | |||
| β-actin | NM_205518.1 | CACCACAGCCGAGAGAGAAA | 215 |
| CACAGGACTCCATACCCAAGAA | |||
| TBP | AF221563 | AGCTCTGGGATAGTGCCACAG | 134 |
| ATAATAACAGCAGCAAAACGCTTG |
IL-1β, interleukin 1β; IL-6, interleukin 6; TNF-α, tumor necrosis factor α; TLR4, toll-like receptor; NF-κB, nuclear factor kappa-B; IFN-γ, interferon γ ; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; TBP, TATA-binding protein.