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. 2021 Sep 23;12:713485. doi: 10.3389/fimmu.2021.713485

Table 7.

Effects of dietary treatment on antioxidant capacity in 7- and 14-day-old broilers.

Items GML levels (mg/kg feed) SEM P-value
CON 300 600 900 1200 Linear Quadratic
Serum, 7 d of age
MDA (nmol/ml) 5.88 5.29 5.99 5.67 5.08 0.19 0.353 0.506
SOD (U/ml) 67.41b 71.9ab 65.4b 82.87ab 93.84a 3.84 0.196 0.009
T-AOC (mmol/ml) 0.56 0.54 0.57 0.58 0.63 0.02 0.143 0.449
Serum, 14 d of age
MDA (nmol/ml) 6.55a 4.64b 4.07b 4.51b 4.95b 0.22 0.012 0.001
SOD (U/ml) 75.48b 84.33ab 89.43ab 101.29ab 116.44a 4.31 0.001 0.534
T-AOC (mmol/ml) 0.41b 0.47ab 0.49ab 0.52a 0.52a 0.01 0.002 0.272
Jejunum, 7 d of age
MDA (nmol/mgprot) 0.41 0.49 0.52 0.44 0.57 0.03 0.206 0.955
SOD (U/mgprot) 2.62c 3.35bc 3.84ab 3.6ab 4.23a 0.12 <0.001 0.225
T-AOC (mmol/mgprot) 0.08b 0.11ab 0.13ab 0.12ab 0.18a 0.01 0.010 0.898
Jejunum, 14 d of age
MDA (nmol/mgprot) 0.85a 0.6ab 0.56b 0.55b 0.56b 0.03 0.004 0.025
SOD (U/mgprot) 3.72 3.77 3.76 4.01 3.50 0.11 0.855 0.399
T-AOC (mmol/mgprot) 0.09 0.08 0.08 0.08 0.07 0.01 0.335 0.678

MDA, malondialdehyde; SOD, superoxide dismutase; T-AOC, total antioxidant capacity; mgprot, mg of protein; SEM, total standard error of mean.

Values were expressed as least squares means ± SEM (n = 12).

a,b,cDifferent superscripts in the same line indicate significant differences according to Tukey’s multiple comparisons (P < 0.05).