Table 7.
Effects of dietary treatment on antioxidant capacity in 7- and 14-day-old broilers.
| Items | GML levels (mg/kg feed) | SEM | P-value | |||||
|---|---|---|---|---|---|---|---|---|
| CON | 300 | 600 | 900 | 1200 | Linear | Quadratic | ||
| Serum, 7 d of age | ||||||||
| MDA (nmol/ml) | 5.88 | 5.29 | 5.99 | 5.67 | 5.08 | 0.19 | 0.353 | 0.506 |
| SOD (U/ml) | 67.41b | 71.9ab | 65.4b | 82.87ab | 93.84a | 3.84 | 0.196 | 0.009 |
| T-AOC (mmol/ml) | 0.56 | 0.54 | 0.57 | 0.58 | 0.63 | 0.02 | 0.143 | 0.449 |
| Serum, 14 d of age | ||||||||
| MDA (nmol/ml) | 6.55a | 4.64b | 4.07b | 4.51b | 4.95b | 0.22 | 0.012 | 0.001 |
| SOD (U/ml) | 75.48b | 84.33ab | 89.43ab | 101.29ab | 116.44a | 4.31 | 0.001 | 0.534 |
| T-AOC (mmol/ml) | 0.41b | 0.47ab | 0.49ab | 0.52a | 0.52a | 0.01 | 0.002 | 0.272 |
| Jejunum, 7 d of age | ||||||||
| MDA (nmol/mgprot) | 0.41 | 0.49 | 0.52 | 0.44 | 0.57 | 0.03 | 0.206 | 0.955 |
| SOD (U/mgprot) | 2.62c | 3.35bc | 3.84ab | 3.6ab | 4.23a | 0.12 | <0.001 | 0.225 |
| T-AOC (mmol/mgprot) | 0.08b | 0.11ab | 0.13ab | 0.12ab | 0.18a | 0.01 | 0.010 | 0.898 |
| Jejunum, 14 d of age | ||||||||
| MDA (nmol/mgprot) | 0.85a | 0.6ab | 0.56b | 0.55b | 0.56b | 0.03 | 0.004 | 0.025 |
| SOD (U/mgprot) | 3.72 | 3.77 | 3.76 | 4.01 | 3.50 | 0.11 | 0.855 | 0.399 |
| T-AOC (mmol/mgprot) | 0.09 | 0.08 | 0.08 | 0.08 | 0.07 | 0.01 | 0.335 | 0.678 |
MDA, malondialdehyde; SOD, superoxide dismutase; T-AOC, total antioxidant capacity; mgprot, mg of protein; SEM, total standard error of mean.
Values were expressed as least squares means ± SEM (n = 12).
a,b,cDifferent superscripts in the same line indicate significant differences according to Tukey’s multiple comparisons (P < 0.05).