. 1999 Dec;19(12):8513–8525. doi: 10.1128/mcb.19.12.8513

TABLE 1.

Primers used for RT-PCR analysis

Site	Size of first-round PCR product (bp)	First-round PCR primers		Second-round nested PCR primers
Site	Size of first-round PCR product (bp)	Forward	Reverse	Forward	Reverse
MX6^a	520	tcctcctgactctgcctactg	cactccttctgttattaatatgcc	ggaaggagcatcaggagtt	atgcacagtcaagcaggag
MX3^a	1,210	tgtgaagagcccttccttg	gttgcacgcctttaactgat	gtaacctttacttctcaattcag	cagaagaggagtggccacaaag
X1^b	541	gagatacatttatttgctca	gacttagtttggtttcttta	agtcttttctcaaggtcctaa	tatgtttacattacaggtggc
X2^b	582	gagtttctttctgtgccaccc^d	ccgtccagtggtctgttctg^d	ctgtgccacccactgaaa	gtcgtctggaccaacaagg
X3^b	561	ggggtttctatctgatctcca	ctccatccaagttctttctgg	ggcaagtcaataaagcactcc	cactggcaaggtgaatagcat
X4^b	722	aactgggtcttcagcgtgat	catgcaactccagggcatatt	aaatgacccgaggatcaacat	taagcagcaaacaccataagc
1^c	597	3003–3023	3581–3600	3267–3286	3445–3465
2^c	221	5928–5947	6129–6149	5941–5958	6084–6103
3^c	628	10867–10886^d	11478–11495^d	10920–10938	11419–11439
4^c	964	11365–11384^d	12310–12329^d	11384–11404^d	12288–12308^d
5^c	830	12310–12329	13120–13140	12348–12366^d	13079–13096^d
6^c	809	13120–13140^d	13907–13929^d	13141–13162	13892–13913
7^c	516	13907–13929^d	14403–14423^d	13941–13961	14367–14387
8^c	521	14403–14423^d	14904–14924^d	14412–14433	14880–14901
9^c	1,284	14810–14827	16074–16094	14822–14842	15989–16009
10^c	520	16304–16323	16807–16824	16327–16344^d	16783–16800^d
11^c	982	16831–16848	17794–17813	16895–16915	17674–17694
12^c	878	17794–17813^d	18653–18672^d	17874–17894^d	18615–18635^d
13^c	1,022	18653–18672^d	19656–19675^d	18724–18744	19605–19625
14^c	1,116	19656–19675	20753–20772	19778–19798	20657–20677
15^c	962	25554–25574	26496–26516	25749–25769	26451–26471

Primers (5′ to 3′) derived from sequence with accession no. AJ010350.

Primers (5′ to 3′) derived from sequence with accession no. U41394, U41395, and U41396.

Primer coordinates derived from sequence with accession no. X99946.

PCR annealing temperature was 58°C rather than 55°C.