TABLE 2.
Characteristics of ΔDXPas34 transgenic ES cell lines
| Line | YAC transgene | Copy no.b | Phenotype in ES cellsc
|
|||
|---|---|---|---|---|---|---|
| Undifferentiated, expression in transgene:
|
Differentiated (Xist RNA coating in cis) | |||||
| 5′ Xist | Xist | 3′ Xist | ||||
| L412a | PA-2 (460 kb) | 2 | + | + | + | + |
| L117a | PA3 (320 kb) | 5–8 | + | + | + | + |
| LF1 | PA3 F1n Δ34.3 | 3–4 | − | − | − | + |
| LP5 | PA-2 Δ34.1 | 2–3 | − | − | − | + |
| LP1 | PA-2 Δ34.1 | 1 | − | − | − | − |
| LP6 | PA-2 Δ34.1 | 1 | − | − | − | − |
| LP17 | PA-2 Δ34.1 | 1 | − | − | − | − |
a
Reference 20 and this study.
b
Copy number of the transgene determined as described in Materials and Methods.
c
Assessed by RNA FISH with probes mx8 (5′ Xist), λ510 (Xist) and sites 2 and 3 (3′ Xist; see figure 1A).