Figure 1.

CSFV infection affects the metabolism of lactic acid and pyruvic acid in pigs and PK-15 cells. (A and B) SPF Bana pigs infected with CSFV at different time points, and the blood was collected from veins. The plasma concentrations of lactic acid (A) and pyruvic acid (B) were measured by spectrophotometric assays. (C and D) PK-15 cells were infected with CSFV at a MOI of 0.1 and measured lactate (C) and pyruvate (D) concentrations by spectrophotometric assays in the culture supernatant at 24 and 48 h. (E and F) PK-15 cells were transfected with siLDHB prior infected with CSFV at a MOI of 0.1 and measured lactate (E) and pyruvate (F) concentrations by spectrophotometric assays in the culture supernatant at 24 and 48 h. All measurements were made in triplicates. Error bars indicate the mean (±SD) of 3 independent experiments. *, P < 0.05; **, P < 0.01; and ***, P < 0.001 (one-way ANOVA)