Figure 2.

Increased survival of L1 arrest in double mutant ire-1;rnf-5 compared to ire-1 single mutant. (A) Survival rates of rnf-5;ire-1 double mutant are higher than ire-1 single mutant. (B) Survival rates of atg-3 single mutant and atg-3;rnf-5 double mutant are lower than wild-type and rnf-5 single mutant. (C) Survival rates of atg-3;ire-1 double mutant are lower than single atg-3 or ire-1 mutants. (D) Survival rates of atg-3(bp412);rnf-5(tm794);ire-1(v33) triple mutant are lower than rnf-5(tm794);ire-1(v33) double mutant. Animals were incubated in M9 buffer without food for the indicated time points and then dropped on plates with OP50 bacteria (food). The percentage of worms developed into L4 larvae and adults during 3 days of re-feeding is shown. Errors bars are the SEs at each time point indicated. (E) DIC images at day 10 of L1 arrest. Arrows point to dying cells