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RAB33B-ATG16L1 interaction is essential for autophagy. (A) atg16l1−/− MEF transiently expressing EGFP or the indicated EGFP-ATG16L1 constructs were incubated in EBSS for 2 h, lysed and subjected to immunoblot analysis. (B) Normalized ratio of SQSTM1:ACTA1 from immunoblots in (A). Data are represented as mean ± SD, from 3 independent experiments. WT: wild type, NN: ATG16L1N206A,N209A. *p ≤ 0.05, **p ≤ 0.01 (Student’s t-Test)
