Fig 2. Tissue macro-categories show both HSA21 and non-HSA21 genes that are consistently DE.

a) Histogram of DE genes’ occurrences. Genes in the right 5% are in the right of the red dot line, corresponding to genes found DE in at least 4 comparisons b) Entropy distribution across consistently DE genes. Each dot is a gene, the horizontal bar indicates the median entropy for each group. All genes consistently DE in iPSCs/ESCs were also found DE in at least another macro-category up to 4 other macro categories. Gene consistently DE in fibroblasts were also found DE in one or two macro-categories. Similarly, genes consistently DE in placenta/amnios were found DE also in 2 or 3 other macro categories. For brain and blood tissue we did detect a fraction specifically DE only in one of the two macro-categories, however, also in this case we detected genes DE in more macro-categories (1 to 4 for brain; 1 to 5 for blood). In black are genes that are consistently DE at the tissue level in more than one macro-category. In white, genes that are not consistently DE in any specific macro categories but are consistently DE across tissue. c) Bipartite graph showing the tissue macro-categories where we could find consistently DE genes at the tissue level. For each tissue macro-category, we plotted the frequency of the number of occurrences. The red dotted line indicates the minimal number of occurrences chosen for a gene to be DE in a given tissue macro-category. Genes mapping on HSA21 are represented by squares, otherwise by circles. Node size is proportional to the log₂ of the number of occurrences across all comparisons. Of note, most consistently DE genes are HSA21. d) Barplot for the percentage (%) of HSA21 genes for each entropy level. The dotted line indicates the percentage (%) of HSA21 genes in the top-500 DE genes. e) Barplot showing the percentage (%) of tissue specific genes for each entropy level, considering all the top 500 genes f) As in e). but, only considering the top 500 genes mapping on HSA21.