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. 2021 Oct 7;16(10):e0257824. doi: 10.1371/journal.pone.0257824

Table 1. Composition of buffer solutions used for Ussing chamber experiments (if not stated otherwise, all chemicals were obtained from Merck, Darmstadt, Germany).

All chemicals were diluted in aqua destillata.

Mucosal buffer [mM] Serosal buffer [mM]
NaCl 119.6 119.6
KCl 5.4 5.4
HCl 0.4 0.4
MgCl2 * 6 H2O 1.2 1.2
CaCl2 * 2 H2O 1.2 1.2
NaHCO 3 15.0 15.0
Na 2 HPO 4 1.2 1.2
NaH 2 PO 4 0.3 0.3
Mannitol 20.0 10.0
Glucose - 10.0
Indomethacin (Sigma-Aldrich, Schnelldorf, Germany) 0.01 0.01