Table 1. Composition of buffer solutions used for Ussing chamber experiments (if not stated otherwise, all chemicals were obtained from Merck, Darmstadt, Germany).
Mucosal buffer [mM] | Serosal buffer [mM] | |
---|---|---|
NaCl | 119.6 | 119.6 |
KCl | 5.4 | 5.4 |
HCl | 0.4 | 0.4 |
MgCl2 * 6 H2O | 1.2 | 1.2 |
CaCl2 * 2 H2O | 1.2 | 1.2 |
NaHCO 3 | 15.0 | 15.0 |
Na 2 HPO 4 | 1.2 | 1.2 |
NaH 2 PO 4 | 0.3 | 0.3 |
Mannitol | 20.0 | 10.0 |
Glucose | - | 10.0 |
Indomethacin (Sigma-Aldrich, Schnelldorf, Germany) | 0.01 | 0.01 |