Fig 1. Size Exclusion Chromatography analyses of CwlD, GerS, and the CwlD:GerS complex.

Purified CwlD_Δ25-His₆ (A), GerS_Δ22-His₆ (B), and the CwlD_Δ25-His₆:GerS_Δ22 complex (C) were analyzed by size exclusion chromatography. mAU corresponds to the UV absorbance measurements (A₂₈₀) during the protein elution. The estimated molecular weights of the protein species are indicated at the top of each peak. The input sample is derived from the co-affinity purification high imidazole elution fraction. This input sample as well as the fractions (0.5 mL) from the indicated elution volumes were analyzed by SDS-PAGE and Coomassie staining. Dashed grey lines indicate the fractions collected. The data shown are from one biological replicate, which is representative of three biological replicates performed independently. The molecular weight (MW) marker is shown on the left-hand side of the gel.