Fig 4. Zn2+ stabilizes the CwlD:GerS complex.
Purified wild-type CwlD:GerS (A) and CwlDH49A:GerS (B) complexes were analyzed by size exclusion chromatography after treatment with a five-fold excess of Zn2+Cl2 (green traces) or 1 mM EDTA (red traces) for 1 hr. mAU corresponds to the UV absorbance measurements (A280) during the protein elution. Fractions (0.5 mL) corresponding to the indicated elution volumes were analyzed by SDS-PAGE. The numbers at the bottom of the gels correspond to the apparent MW of each peak. The data shown are from one biological replicate, which is representative of three biological replicates performed independently.