Fig 5. Elemental analyses of CwlD and CwlD:GerS complex.
Elemental analyses of Zn2+ in CwlD:GerS complexes corresponding to His-tagged wild-type CwlD:GerS and CwlDH49A:GerS, and individual His-tagged proteins (wild-type CwlD, GerS and CD2761 and CwlDH49A). Elemental analyses were performed on affinity-purified proteins produced in E. coli in the absence of Zn2+ supplementation that were further purified using size exclusion chromatography (also in the absence of Zn2+ supplementation). Each dot represents data from a given biological replicate. Three independent protein purifications were analyzed with the exception for CwlDH49A and CD2761, for which two independent protein purifications were performed. Bars represent the mean of the three (or two) independent experiments. Standard deviation is shown. Statistical significance was determined using a one-way ANOVA and Tukey’s test. **, p < 0.005; **** p < 0.0001.