FIG. 3.

Structural features of the SCAN domain required for self-association. A schematic representation of ZNF174 is shown at the top. The regions of the SCAN domain that were cloned into GAL4 BD and VP16 AD expression plasmids are indicated below. The first construct contains the full-length wild-type SCAN domain. The next two constructs represent smaller regions of the SCAN domain, with each one expressing individual predicted α-helices. The last construct contains the full-length SCAN domain in which two conserved leucines have been mutated to prolines. α-helical regions within the SCAN domain are indicated (H) below each construct. Relative levels of CAT activity between pairs of the expression constructs when tested by two-hybrid analysis are shown on the right.