Table of Materials
| Name of Material/Equipment | Company | Catalog Number | Comments/Description |
|---|---|---|---|
| Antibodies | |||
| TNNT2 | Abcam | ab8295 | Mouse; 1:200 |
| HAND1 | Abcam | ab196622 | Rabbit; 1:200 |
| HAND2 | Abcam | ab200040 | Rabbit; 1:200 |
| WT1 | Abcam | ab89901 | Rabbit; 1:200 |
| TJP1 | Invitrogen | PA5–19090 | Goat; 1:250 |
| NFAT2 | Abcam | ab25916 | Rabbit; 1:100 |
| PECAM1 | DSHB | P2B1 | Rabbit; 1:50 |
| THY1 | Abcam | ab133350 | Rabbit; 1:200 |
| VIM | Abcam | ab11256 | Goat; 1:250 |
| Alexa Fluor 488 Donkey anti- mouse | Invitrogen | A-21202 | 1:200 |
| Alexa Fluor 488 Donkey anti- rabbit | Invitrogen | A-21206 | 1:200 |
| Alexa Fluor 594 Donkey anti- mouse | Invitrogen | A-21203 | 1:200 |
| Alexa Fluor 594 Donkey anti- rabbit | Invitrogen | A-21207 | 1:200 |
| Alexa Fluor 647 Donkey anti- goat | Invitrogen | A32849 | 1:200 |
| Media & Reagents | |||
| Essential 8 Flex Medium Kit | Gibco | A2858501 | Medium containing 1% penicillin-streptomycin |
| DMEM/F12 | Gibco | 10566016 | |
| Penicillin-Streptomycin | Gibco | 15140122 | |
| Matrigel GFR | Corning | CB40230 | |
| ReLeSR | Stem Cell Technologies | NC0729236 | |
| Accutase | Innovative Cell Technologies | NC9464543 | |
| Thiazovivin | Millipore Sigma | SML1045 | |
| Phosphate Buffer Solution | Gibco | 10010049 | |
| Phosphate Buffer Solution (10x) | Gibco | 70011044 | |
| RPMI 1640 | Gibco | 11875093 | |
| B-27 Supplement | Gibco | 17504–044 | |
| B-27 Supplement (Minus Insulin) | Gibco | A1895601 | |
| CHIR-99021 | Selleck | 442310 | |
| BMP-4 | Gibco | PHC9534 | |
| Activin A | R&D Systems | 338AC010 | |
| WNT-C59 | Selleck | NC0710557 | |
| Dimethyl Sulfoxide | Millipore Sigma | D2650 | |
| DAPI | Thermo Scientific | 62248 | 1:1000 |
| Fluo4-AM | Invitrogen | F14201 | |
| Paraformaldehyde | MP Biomedicals | IC15014601 | Powder dissolved in PBS Buffer – use at 4% |
| Glycine | Millipore Sigma | 410225 | |
| Normal Donkey Serum | Millipore Sigma | S30–100mL | |
| Triton X-100 | Millipore Sigma | T8787 | |
| Bovine Serum Albumin | Bioworld | 50253966 | |
| D-(-)-Fructose | Millipore Sigma | F0127 | |
| Glycerol | Millipore Sigma | G5516 | |
| VECTASHIELD Vibrance Antifade Mounting Medium | Vector Laboratories | H170010 | |
| Polybead Microspheres | Polysciences, Inc. | 73155 | 90 μm |
| Trypan Blue Solution | Gibco | 1525006 | |
| Other | |||
| 96-well Clear Ultra Low Attachment Microplates | Costar | 07201680 | |
| Moxi Cell Counter | Orflo Technologies | MXZ001 | |
| Moxi Z Cell Count Cassette – Type M | Orflo Technologies | MXC001 | |
| 8 Well chambered cover Glass with #1.5 high performance cover glass | Cellvis | C8–1.5H-N | |
| 15mL Falcon Tubes | Fisher Scientific | 1495970C | |
| 1.5 Microcentrifuge Tubes | Fisher Scientific | 02682002 | |
| 6-Well Flat Bottom Cell Culture Plates | Corning | 0720083 |