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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: J Proteome Res. 2021 Sep 2;20(10):4901–4911. doi: 10.1021/acs.jproteome.1c00549

Figure 4.

Figure 4.

(A) SDS-PAGE analysis of the EVs isolated from 40 μL of human serum using the CD9-HPLC-IAC method followed by silver staining, with a total amount of 1.5 μg of a protein ladder as a reference. (B) Venn diagram showing the overlap of proteins identified in three replicates of EVs isolated from 40 μL of human serum. (C) Heat map of the Pearson correlation coefficient R2 for the comparison of the MS results of the three replicates. (D) Gene ontology analysis of the cellular component, molecular function, and biological process of the identified EV proteins.