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. Author manuscript; available in PMC: 2022 May 10.
Published in final edited form as: Biomacromolecules. 2021 Apr 7;22(5):1990–1998. doi: 10.1021/acs.biomac.1c00096

Figure 4.

Figure 4.

SDS-PAGE analysis comparing protein yield and purity obtained from various methods of A160 (68.06 kDa) purification. Lanes: 1 = ladder; 2 = empty; 3 = cell lysate; 4 = cold spin supernatant from the first round of ITC (CSS1) after lysis; 5 = CSS3 after lysis; 6 = empty; 7 = extraction (BD); 8 = CSS1 after extraction (BD); 9 = ACN precipitation after extraction (BD); 10 = CSS1 after ACN precipitation (BD). Sample volumes were normalized across lanes 5–10, allowing for direct comparisons of ELP yield and purity. Estimated time needed is displayed above each lane. LMWC: smear around 5 kDa can be seen in lanes 7 and 9.