Figure 5. Severe sepsis leads to increased apoptosis of CD8 T_RM.

A) Experimental Design. VacV-GP₃₃ immunized mice containing memory P14 CD8 T cells underwent sham or CLP (moderate or severe) surgery. After 2 days, the mice received an i.v. injection of anti-CD45.2 mAb, tissues were collected 3 minutes later, and cells analyzed by flow cytometry. Frequency of activated caspase3/7⁺ P14 CD8 T cells in the B) PBL, C) spleen, and D) CD103⁺ skin P14 CD8 T_RM 2 days after surgery. Data representative from a single experiment with 3 mice per group. NS = not significant. * = p<0.05; ** = p<0.01; **** = p<0.0001 using 1-way ANOVA corrected for multiple comparisons using Dunn’s test. Error bars represent the standard error of the mean.