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. Author manuscript; available in PMC: 2022 Feb 3.
Published in final edited form as: Sci Signal. 2021 Aug 3;14(694):eaba0245. doi: 10.1126/scisignal.aba0245

Figure 5. Modulation of fear-potentiated startle (FPS) by β-arrestin–biased and G protein-biased δOR agonists.

Figure 5.

(A) Schematic of the experimental paradigm of the FPS test, wherein drugs were administered prior to the tests on the third day. See fig. S5 for the Day 1 acoustic startle test. (B to G) Schematics of the conditions (B and C) in which raw startle amplitudes to “noise” and “light+noise” and % FPS during the test diagrammed in (A) were measured in WT mice (C and D; control, N = 21; SNC80, N = 21) and β-arrestin 2 KO mice (F and G; control, N = 8; SNC80, N = 8) 20 to 30 min after i.p. administration of 20 mg/kg SNC80. (H to J) As described in (B to G), in WT mice 30 to 40 min after i.p. administration of 25 mg/kg TAN-67 (control, N = 8; TAN-67, N = 8). See table S4 for two-way ANOVA and post-hoc multiple comparisons.