Table 1.

Peptide identification from the in-gel digestion of the purified antigen

Protein name/ accession number	Molecular weight (Da)	Position	Peptide sequence	Domain	Sequence coverage (%)	Expected value	Protein score	Retention time (min)	Mass
Nucleoprotein/ NCAP_SARS2	45,598	33–38 62–68 94–100 101–107 144–149 196–203 250–256 294–299 362–369	SGARSK EDLKFPR IRGGDGK MKDLSPR SAAEASK NSTPGSSR QGTDYK DHIGTR TFPPTEPK	NTD NTD NTD NTD NTD SR-rich linker SR-rich linker CTD CTD	14	2.8e−05	71	1.748 10.009 8.99 14.535 11.876 3.106 8.141 4.463 17.195	605.3003 905.3679 702.3656 846.4745 663.2919 805.4151 711.3777 699.3434 916.4878
Spike Glycoprotein/ SPIKE_SARS2	141,088	35–41 196–202 238–246 305–310 409–417 458–466 530–535 568–577 815–825 987–995 1020–1028 1039–1045	GVYYPDK NIDGYFK FQTLLALHR SFTVEK QIAPGQTGK KSNLKPFER STNLVK DIADTTDAVR RSFIEDLLFNK VEAEVQIDR ASANLAATK RVDFCGK	NTD NTD NTD NTD RBD RBD SD1 SD1 FP – HR HR	7	3.5e−05	70	15.271 14.761 11.536 8.141 7.293 7.293 5.765 8.537 8.537 8.537 14.535 16.515	840.3916 855.4207 1099.5145 711.3777 898.4402 559.2666 660.3676 538.2224 1381.5842 1059.4718 846.4745 824.4274
Membrane/ VME1_SARS2	25,130	43–50 151–158 175–180 181–186	NRFLYIIK IAGHHLGR TLSYYK LGASQR	–	12	0.0031	51	19.514 15.836 7.01 10.065	1065.5635 859.4678 774.3603 632.3762
ORF3a/ AP3A_SARS2	31,103	22–30 62–67 69–75 193–198	DATPSDFVR IITLKK WQLALSK WESGVK	–	10	0.0067	47	8.537 7.575 14.535 6.387	1007.4616 716.3357 846.4745 704.3564
ORF8/ NS8_SARS2	13,822	45–52 95–101 116–121	WYIRVGAR LGSLVVR VVLDFI	–	17	0.011	45	11.536 10.009 6.387	1021.46 371.655 704.3564
Envelope/ VEMP_SARS2	8360	62–69 64–69 70–75	VKNLNSSR NLNSSR VPDLLV	CTD	18	0.012	38	7.575 14.705 2.653	916.4513 689.3618 655.3016

Placeholder TextPeptides with 7 amino acids or higher are considered as plausible sequences for further analyses