Table 2.

Primer pairs used for qRT-PCR.

Primer sequence
Cox-1	F: 5′-TGACTATCTGGCGGGTGACT-3′ R: 5′-CTTGCTGGACATTGGGTTCT-3′
Gapdh	F: 5′-ACAGCAACAGGGTGGTGGAC-3′ R: 5′-TTTGAGGGTGCAGCGAACTT-3′
Real-time PCR condition
Step	Temperature (°C)	Time	Cycle
Initial denaturation	95	10 min	1
Denaturation	95	15 sec	40
Annealing and Extension	60	1 min
Melt curve stage	95	15 sec	1
	60	1 min