Figure 4.

Curcumin treatment attenuates caspase-1 positive microglia in the ischemic cortex, corpus, and striatum at 21 days after MCAO. Representative immunofluorescence costaining for caspase-1 and Iba-1 markers in brain sections obtained from the curcumin- or vehicle-treated groups. (a) Costaining for Iba-1 (red) and caspase-1 (green) in the perilesion cortex, corpus callosum, and striatum. Scale bar in the figures of 1-3 columns from left = 20 μm, and scale bar in the figure of the 4th column from left = 10 μm. Arrows indicate caspase-1⁺Iba-1⁺ microglia. (b) Schematic diagram illustrating the anatomical location of images in the perilesion cortex (blue), corpus callosum (red), and striatum (green). Quantification of the percentage of caspase-1⁺Iba-1⁺ cells among total Iba-1⁺ cells in perilesion cortex (c), corpus callosum (d), and striatum (e). Values are the mean ± SEM. ^∗∗∗p < 0.001. n = 8 mice per group. One-way ANOVA followed by Bonferroni post hoc test.