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. 2021 Sep 30;2021:1552127. doi: 10.1155/2021/1552127

Figure 5.

Figure 5

Curcumin inhibits microglial pyroptosis in lipopolysaccharide- (LPS-) and ATP-stimulated primary microglial cells. Primary microglia were extracted from the cortex of newborn C57BL/6J mice. Microglia were treated with vehicle or curcumin (12.5 μM) in the presence or absence of LPS (100 ng/mL) for 24 hours followed by ATP (1 mM) stimulation for further 3 hours. (a, b) Representative western blot of pyroptosis-related proteins. Quantitative analysis of western blot data for NLRP3 (c), pro-caspase-1 (d), cleaved caspase-1 (e), GSDMD-FL (f), GSDMD-N (g), pro-IL-1β (h), IL-18 (i), pro-IL-18 (j), and IL-18 (k). All the data are means ± SEM. n = 3 per group. Samples were collected from three independent experiments. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001, one-way ANOVA followed by Bonferroni post hoc test. The mRNA expression of NLRP3 (l), caspase-1 (m), IL-18 (n), and IL-1β (o) was examined by real-time PCR. All the data are means ± SEM. n = 3 per group. Samples were collected from three independent experiments. ∗∗p < 0.01 and ∗∗∗p < 0.001, one-way ANOVA followed by Bonferroni post hoc test.