Figure 8.

Curcumin treatment inhibits the stroke-induced activation of NF-κB pathway in vivo and in vitro. In vivo, curcumin (150 mg/kg) was intraperitoneally injected into mice after reperfusion and once per day for 7 days after MCAO. Brain samples were collected from the ipsilateral peri-infarct regions 21 days after cerebral ischemia. In vitro, primary microglia were induced to pyroptosis with LPS plus ATP after pretreatment with curcumin or vehicle for 24 hours. Representative western blot of NF-κB pathway-related proteins in vivo (a) and in vitro (b). Quantitative analysis of western blot data for IκBα (c, l), phos-IκBα (d, m), the relative ratio of phos-IκBα vs. total IκBα (e, n), p65 (f, o), phos-p65 (g, p), the relative ratio of phos-p65 vs. total p65 (h, q), IKKα/β (i), phos-IKKα/β (j), and the relative ratio of phos-IKKα/β vs. total IKKα/β (k). Values are the mean ± SEM. In vivo, n = 8 mice per group, 1 band/mouse. In vitro, samples were collected from three independent experiments. n = 3 per group. ^∗∗p < 0.01 and ^∗∗∗p < 0.001, one-way ANOVA followed by Bonferroni post hoc test.