Table 3.
The information of primary and secondary antibodies.
| Primary antibody | Source | Dilution |
|---|---|---|
| GSDMD | ARG41404, Arigo Biolaboratories | 1 : 500 |
| IL-1β | ab234437, Abcam | 1 : 1000 |
| IL-18 | #57085, Cell Signaling | 1 : 1000 |
| Caspase-1 | #89332, Cell Signaling | 1 : 1000 |
| NLRP3 | ab214185, Abcam | 1 : 100 |
| phos-IKKα/β (Ser176/180) | #2697, Cell Signaling | 1 : 1000 |
| IKKα/β | ab178870, Abcam | 1 : 1000 |
| phos-p65 (Ser536) | #3036, Cell Signaling | 1 : 1000 |
| p65 | #8242, Cell Signaling | 1 : 1000 |
| phos-IκBα (Ser32) | #2859, Cell Signaling | 1 : 1000 |
| IκBα | #4812, Cell Signaling | 1 : 1000 |
| GAPDH | ab181602, Abcam | 1 : 10000 |