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. 2021 Jul 5;58(10):4921–4943. doi: 10.1007/s12035-021-02439-1

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Fig. 8 — LTP at PF-Purkinje cell synapse is impaired in MUT rats. A LTP induced in WT rats by stimulation of the PFs in the molecular layer at 1 Hz for 10 min induces LTP in WT rat cerebellar slices (green circles, n = 14 slices from 6 rats), but not in cerebellar slices from MUT rats (red triangles, n = 16 slices from 6 rats). Each data point represents the average of two successive test responses. The vertical gray bar indicates the period of 1-Hz stimulation. Typical traces show EPSPs before (black line) and after (green line for WT, red line for MUT) 1-Hz stimulation in slices from WT (left) and MUT (right) rats (data are shown as mean ± SEM; ***p < 0.001, two-way repeated measures ANOVA with Bonferroni post hoc test [RM ANOVA]). B Baseline and potentiated synaptic strength in PF-PC synapses. Graph depicts amplitude of fEPSP evoked from WT slices (green, n=14 from 6 rats) and MUT slices (red, n=13 from 6 rats) by stepwise increase in the stimulus from 5 to 100 μA. WT slices show increased amplitudes after 1 h LTP (upper left panel, ***p < 0.001, two-way RM ANOVA). MUT slices show no increase in amplitudes after 1-h LTP (upper right panel, ns). Comparison of amplitudes between WT and MUT slices before LTP (lower left panel, *p < 0.05, two-way RM ANOVA) and after LTP (lower right panel, **p < 0.01, two-way RM ANOVA). C Changes in the synaptic responses during the 1-Hz stimulation trains. Normalized fEPSP amplitude in WT slices (green circles, n=14 slices from 6 rats) increased significantly compared with MUT slices (red triangles, n=14 slices from 6 rats). Each data point represents one fEPSP response as recorded every second and normalized to the amplitude of first response (data are shown as mean ± SEM, **p < 0.01, two-way ANOVA, last 100 s). D fEPSP average amplitude of the first response in WT and MUT slices (same data set as panel C; shown as mean ± SEM, ns, Mann-Whitney test). E Ratio of average amplitude of the last five responses (#596–600) divided by the average of the first five responses (#1–5) in WT and MUT slices (data are shown as mean ± SEM, ***p < 0.001, Mann-Whitney test)