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. 2021 Jul 5;58(10):4921–4943. doi: 10.1007/s12035-021-02439-1

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Fig. 9 — LTD at CF-PC synapses is impaired in MUT rats. A Stimulating the CFs in the granule cell layer at 1 Hz for 10 min induces LTD in WT cerebellar slices (green circles, n = 10 slices from 5 rats), but not in cerebellar slices from MUT rats (red triangles, n = 14 slices from 6 rats). Each data point represents the average of two successive test responses. The vertical gray bar indicates the period of 1-Hz stimulation. Representative traces depict fEPSPs before (black line) and after (green line for WT and red line for MUT) application of the 1-Hz LTD protocol in WT and MUT slices (left and right inset, respectively) (data are shown as mean ± SEM; **p < 0.01, ***p < 0.001, two-way repeated measures ANOVA with Bonferroni post hoc test [RM ANOVA]). B Baseline and depressed synaptic strength in CF-PC synapses. Graph depicts amplitude of fEPSP evoked from WT slices (green, n=8 from 5 rats) and MUT slices (red, n=12 from 6 rats) by stepwise increase in the stimulus from 5 to 100 μA. Note the decreased baseline CF synaptic strength and lack of LTD in MUT slices after 1-Hz stimulus. WT slices show decreased amplitudes after 1-h LTD (upper left panel, *p < 0.05, two-way RM ANOVA). MUT slices show no changes in amplitudes after 1-h LTD (upper right panel, ns). Comparison of amplitudes between WT and MUT slices before LTD (lower left panel, ***p < 0.001, two-way RM ANOVA) and after LTD (lower left panel, *p<0.05, two-way RM ANOVA). C Changes in the synaptic responses during the 1-Hz stimulation trains. fEPSP amplitude in WT slices (green circles, n=10 slices from 5 rats) did not change significantly compared with MUT slices (red triangles, n=12 slices from 6 rats). Each data point represents one fEPSP response as recorded every second and normalized to the amplitude of first response (data are shown as mean ± SEM, ns, two-way ANOVA, last 100 s). D Average amplitude of the first fEPSP response in WT and MUT slices (data are shown as mean ± SEM, ns, Mann-Whitney test). E Ratio of average amplitude of the last five fEPSP responses (#596–600) divided by the average of the first five responses (#1–5) in WT and MUT slices (data are shown as mean ± SEM, ns, Mann-Whitney test)