Fig. 2.
Therapeutic window of NIR laser treatment in rat organotypic hippocampal slices exposed to OGD. A Qualitative analysis representing organotypic hippocampal slice in control condition or exposed to 7.42 J/cm2 immediately or 30 min or 60 min after 30 min OGD and 24 h later incubated with PI for fluorescence detection. B Quantitative analysis of CA1 PI fluorescence expressed as percentage of OGD toxicity. Data are expressed as percentage of OGD-induced PI fluorescence in CA1 region. Bars represent the mean ± SEM of at least four experiments run in quadruplicate. **P < 0.01 vs. OGD (ANOVA + Tukey’s w test)