Fig. 2.

NEAT1 was a direct target gene of miR-128-3p. a Linear regression analysis between expression levels of NEAT1 and miR-128-3p in glioma tissues. b–e Effects of lentiviral transfections were confirmed by qRT-PCR. An inversely relationship was observed between expression levels of NEAT1 and miR-128-3p in glioma cells. *p < 0.05, ***p < 0.001 VS shCtrl group; #p < 0.05, ##p < 0.01, ###p < 0.001 VS pLV-Ctrl group. f The hypothesized wild-type or mutational miR-128-3p binding sites in ITGA5. g Luciferase activities in U87MG cells co-transfected with vector NEAT1-3′UTR-WT or NEAT1-3′UTR-MUT and miR-128-3p mimics or NC mimics was analyzed by Luciferase reporter assay. ***p < 0.001. h Specific association between NEAT1 and miR-128-3p following a RNA pull-down assay in U87MG cells. ***p < 0.001. i, j RIP assay indicating both of NEAT1 and miR-128-3p were enriched by anti-AGO2 in U87MG cells, whether miR-128-3p was overexpressed beforehand or not. *p < 0.05, ***p < 0.001 VS anti-IgG group