Fig. 6.

miR-128-3p mediated the pro-oncogenic effects of ITGA5. a The predicted wild-type or mutated miR-128-3p binding sites in ITGA5. b Luciferase activities in U87MG cells co-transfected with vector ITGA5-3′UTR-WT or ITGA5-3′UTR-MUT and miR-128-3p mimics or NC mimics as determined with luciferase reporter assay. ***p < 0.001. c The effects of NEAT1 and miR-128-3p on the proliferation of U87MG and U251MG cells as examined with CCK-8 assays. d The effects of NEAT1 and miR-128-3p on the migration and invasion of U87MG and U251MG cells as evaluated with transwell assays. e, f The effects of NEAT1 and miR-128-3p on apoptosis and cell cycle progression of U87MG and U251MG cells were determined by flow cytometry assays. *p < 0.05, **p < 0.01, ***p < 0.001 VS pLV-Ctrl group; #p < 0.05, ##p < 0.01, ###p < 0.001 VS pre-miR-128-3p group