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. Author manuscript; available in PMC: 2022 Sep 9.
Published in final edited form as: Cells Tissues Organs. 2021 Sep 9;210(4):301–310. doi: 10.1159/000517622

Figure 1. Overview of Cell Seeding and Lung Culture Methods.

Figure 1.

Rat lungs were first decellularized over a 3 day period. The resulting scaffolds were either statically or rotationally seeded for 3 hours. Statically-seeded lungs were transferred into the main chamber of the perfusion-ventilation system and left at 37° C overnight. Alternatively, to encourage cell distribution, decellularized lungs were also placed in a 50 mL conical tube (Step 2B, part C) on two cylinders for periodic rotation. A stepper motor (Step 2B, part C) controlled by a programmed Arduino microcontroller rotated one cylinder (Step 2B, part B) and subsequently the lung within the tube systematically every 20 minutes for 3 hours.