FIG. 1.

Expression of exogenous and endogenous PKC isozymes in primary mouse keratinocytes. After 3 days in primary culture, mouse keratinocytes were infected with 50 PFU/cell of either AdFPKCδ or Adβgal (vector) for 48 h. (A) Cell lysates from both vector- and AdFPKCδ-infected cells were collected and subjected to either immunoblotting with anti-PKCδ or anti-FLAG antibodies or immunoprecipitation (Ip) with anti-FLAG antibody and immunoblotted with anti-PKCδ antibodies. (B) The expression of endogenous PKC isozymes α, ζ, ɛ, and η was examined by immunoblotting of cell lysates 48 h after infection with either AdFPKCδ or Adβgal.