Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 7;12:5888. doi: 10.1038/s41467-021-26147-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a The Corelet system enables light-driven oligomerization to probe protein phase behaviour. b BMI1 visualization with immunofluorescence and overexpression. Scale bar is 5 µm. c BMI1-mCh-sspB in HEK293 cells with NLS-Ferritin-Corelet before (t = 0) and after blue light activation (3 min). Scale bar is 5 µm. d FRAP recovery curve of BMI1-mCh-sspB Corelets. Data are presented as mean +/− SD, n = 5 cells. e BMI1 condensates fuse with one another. Arrows indicate condensates about to fuse. Scale bar is 1 µm. f PHC1 visualization with immunofluorescence and overexpression. Scale bar is 5 µm. g PHC1-mCh-sspB in HEK293 cells with NLS-Ferritin-Corelet before (t = 0) and after blue light activation (3 min). Scale bar is 5 µm. h FRAP recovery curve of PHC1-mCh-sspB Corelets. Data are presented as mean +/− SD, n = 8 cells. i PHC1 condensates fuse with one another. Arrows indicate condensates about to fuse. Scale bar is 1 µm. j CBX2 visualization with immunofluorescence and overexpression. Scale bar is 5 µm. k CBX2-mCh-sspB in HEK293 cells with NLS-Ferritin-Corelet before (t = 0) and after blue light activation (3 min). Scale bar is 5 µm. l FRAP recovery curve of CBX2-mCh-sspB Corelets. Data are presented as mean +/− SD, n = 10 cells. m Pearson correlation coefficient between chromatin (stained with Hoechst, example images in Fig. 4) and mCh-sspB (control, n = 93 cells), CBX2-mCh-sspB (in the dark state, n = 83 cells), and CBX2-mCh-sspB (after activation, n = 108 cells). CBX2-mCh-sspB preferentially localizes with chromatin. This colocalization is increased after activation. Statistical significance, indicated by asterisks, was tested with a two-tailed t test, P = 3.0190e−24 for control-Dark and P = 1.0876e−06 for Dark-Activated. Central mark of boxplot represents median, bottom and top edges of the box indicate the 25th and 75th percentiles, respectively, and whiskers extend to the most extreme data points not considered outliers. n RNF2 visualization with immunofluorescence and overexpression. Scale bar is 5 µm. o RNF2-mCh-sspB in HEK293 cells with NLS-Ferritin-Corelet before (t = 0) and after blue light activation (3 min). Scale bar is 5 µm. p FRAP recovery curve of RNF2-mCh-sspB Corelets. Data are presented as mean +/− SD, n = 8 cells. q Partition coefficient of mCh-sspB (control, n = 109 cells), RNF2-mCh-sspB (in the dark state, n = 92 cells), and RNF2-mCh-sspB (after activation, n = 109 cells) on the inactive X-chromosome. Statistical significance, indicated by asterisks, was tested with a two-tailed t test, P = 2.2874e−25 for control-Dark, P = 5.5943e−10 for Dark-Activated. Central mark of boxplot represents median, bottom and top edges of the box indicate the 25th and 75th percentiles, respectively, and whiskers extend to the most extreme data points not considered outliers.