Table 1.

Animal manure samples and sources.

Sample	Sources	Sample ID	Location	Description of location and handling of livestock
Cow dung	Boerdery farm produce	C1	− 26.21779, 27.6414784	A farm producing animal feeds as well as rearing cattle fed on grains and concentrates in Randfontein, Gauteng
	Kates farm	C2	− 26.0990952, 27.9103166	A bed and breakfast farm facility in the outskirts of Johannesburg, Gauteng serving beef and chicken from livestock locally reared in a free-range feeding scheme
	Bosheuvel country estates	C3	− 26.0249182, 27.8929324	A farm in the Muldersdrift area of Johannesburg with an on-site vintage hotel, restaurant, event venues and conference rooms serving locally reared Pinzgauer cattle, pigs, chickens, and sheep fed mostly on mixed vegetables and tubbers
Horse manure	Earth centre	H1	− 26.080926, 27.8747353	A non-profit company situated in Ruimsig, Gauteng specialising in Equine Assisted Programmes for children with disabilities.The horses are fed with a wide range of feed concentrates and probiotics
	Harveston stables	H2	− 26.0990952, 27.9103167	A yard in Honeydew, Gauteng offering horse riding lessons, stabling, kids pony parties, picnics and pony rides. The horses are fed mainly on feed concentrates
	Barent horse stables	H3	− 26.21779, 27.6414783	A plot rearing horses for family leisure in Randfontein, Gauteng. The horses are fed mostly on lucerne
Pig manure	Bosheuvel country estates	P1	− 26.0249182, 27.8929324	A farm in the Muldersdrift area of Johannesburg with an on-site vintage hotel, restaurant, event venues and conference rooms serving locally reared Pinzgauer cattle, pigs, chickens, and sheep fed mostly on mixed vegetables and tubbers
	Bosheuvel country estates	P2	− 26.0249182, 27.8929324
	Country portion farm	P3	− 26.224517, 27.6325603	A farm in Randfontein producing vegetables, poultry, and pork products to supply local restaurants and consumers. The poultry and pigs are fed on corn and sorghum and concentrates