Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 7;12:5890. doi: 10.1038/s41467-021-25957-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — Symphony comprises two algorithms: Symphony compression (a, b) and Symphony mapping (c, d). a To construct a reference atlas, cells (colored shapes) from multiple datasets are embedded in a lower-dimensional space (e.g., PCA), in which dataset integration (Harmony) is performed to remove dataset-specific effects. Shape indicates distinct cell types, and color indicates finer-grained cell states. b Symphony compression represents the information captured within the harmonized reference in a concise, portable format based on computing summary statistics for the reference-dependent components of the linear mixture model. Symphony returns the minimal reference elements needed to efficiently map new query cells to the reference. c Given an unseen query dataset (red circles) and compressed reference, Symphony mapping precisely localizes the query cells to their appropriate locations within the integrated reference embedding (d). Reference cell locations do not change during mapping. e The resulting joint embedding can be used for downstream transfer of reference-defined annotations to the query cells.